Northern blotting
Northern blotting is a laboratorium technique to analyse RNA expression. The experiment takes several steps. First the total amount of RNA is isolated. Then it is separated on fragment length by gel electroforesis. Then it is transferred to nitrocelloluse or nylon filter paper. The filter can then used to search for a particular RNA by several probing techniques, for instance radioactive labeling of the probe. The probe should be complement to the RNA you are looking for. This simple procedure can indicate in which tissues or cell types a particular gene is expressed. In this way a Northern blot is often used for diagnostic purposes. It can also be used to confirm results from other experimental technques like microarray .
Micro Array Analysis: (see Pictorial representation):
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